Picopure rna isolation kit pdf

In this protocol, dnase treatment is recommended to prevent interference by the picopure rna isolation kit arcturus. Choose the high pure rna isolation kit to rapidly isolate intact total rna from a broad range of research sample materials, including cultured cells, mammalian. Refer to the affymetrix manual about 2x hybridization buffer. Arcturus picopure rna isolation kit is used for extraction and purification of. Determine rna concentration using either a nanodrop spectrophotometer or an available spectrophotometer in your lab. Arcturus picopure rna isolation kit user guide for research use only. Not intended for any animal or human therapeutic or diagnostic use. Isolation kit is optimized for use with cells acquired using laser capture microdissection lcm on applied biosystems. The applied biosystems picopure rna isolation kit from. Rna isolation from picoscale samples with the picopure niehs. Isolation of high quality rna from single mammalian cell. Rna isolation from laser microdissected tissue with the. Rtpcr of gapdh from isolated total rna from 100 lcm captured cells prepared using the. Rna isolation from picoscale samples 1 cell 1025pg 10mg.

Norgens total rna purification micro kit and a competitors arcturus spin column purification kit. Picopure rna isolation kit thermo fisher scientific. The picopure rna isolation kit is optimized for use with cells acquired using laser capture microdissection lcm on capsure hs lcm caps and capsure macro lcm caps but can be used with various cell samples to isolate total cellular rna. The arcturus picopure rna isolation kit is designed to recover highquality total rna. This kit is designed to provide consistent, efficient rna recovery without sacrificing quality. This high dna recovery makes the kit ideal for small samples, such as those prepared by lcm. For an updated list of available kits, please contact technical support at 18008316844. The picopure rna isolation kit contains rna extraction and purification reagents, rna purification columns, and this user guide. This total rna sample was extracted by picopure rna isolation kit molecular devices following manufacturers instructions.

Recommendations for storing rna begin the arcturus picopure rna isolation kit protocol immediately after acquiring cells by lcm. Cell extracts resulting from completion of part i of the protocol may be stored at 80c. The arcturus picopure rna isolation kit is designed to recover highquality total rna consistently from fewer than ten cells, even from a single cell. L buffer 150 capsure hs30 capsure macro lcm cap extractions kit0103 picopure rna isolation kit enough for 40 rna isolations kit0204 capsure macro lcm caps 48 macro caps lcm0211 capsure hs lcm caps 32 hs caps lcm02. According to the product manual, isolated rna can be used in rtpcr, northern blot analysis, dot blot.

The picopure rna isolation kit from arcturus was developed to efficiently isolate total rna from a small number of cells using this kit it is. Picopure rna isolation kit from arcturus biocompare product. Rna purification from trizol samples using picopure. Rna purification from trizol samples using picopure columns. Extract dna for any pcr application dna extracted with the. Product bulletin picopure rna isolation kit picopure rna isolation kit maximize rna recovery from microscopic samples benefi ts enables consistent, effi cient rna recovery lowvolume elution isolate rna from a single cell rapid isolation of rna consistent rna isolation the applied biosystems picopure rna isolation kit from thermo fisher scientifi c enables researchers to. Using the picopure rna isolation kit and other leading products for purifying small rna samples, 100 ng of cy3 dyelabeled rna was bound to columns, washed, and eluted following each manufacturers protocol. Small volume elution allows you to maximize your reco. Picopure dna extraction kit thermo fisher scientific. Arcturusxt tm laser capture microdissection system quick. Providing a stable methodological basis for comparing transcript. Researchers can obtain high recoveries of total cellular. Quantifiable message can be detected from a single cell, and the fluorescence signal is proportional to the cell number for all samples studied. The kit it is optimized for use with cells acquired using laser capture microdissection lcm on capsure hs lcm caps and capsure macro lcm caps but can be used with various cell samples to isolate total cellular rna.